| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.022166 |
| Chromosome: | chromosome 16 |
| Location: | 2060771 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g657150 | NAT35 | N-acetyltransferase; (1 of 1) PTHR23091:SF253 - GCN5-RELATED N-ACETYLTRANSFERASE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAGTTTCGGAGACAGGTGCGTGTGTGCTTGAGGCAGGCTGTGGAGCTG |
| Internal bar code: | TTAATCTCGATGTGCAGAGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3946 |
| LEAP-Seq percent confirming: | 78.5714 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGTGGCCGAATATTGTGC |
| Suggested primer 2: | GGTTGTGAGCGGTTTGAACC |