| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.022181 |
| Chromosome: | chromosome 16 |
| Location: | 6542447 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674450 | (1 of 4) PTHR11266//PTHR11266:SF42 - PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17 // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGGCGGCAAAGGACAGGGAGAGCAAGGAGCGGGAGCGGCCGCTGCTGC |
| Internal bar code: | TGTTCCATTATAGAGGGTCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 343 |
| LEAP-Seq percent confirming: | 44.7368 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGTCCAGCAGCAGTTTG |
| Suggested primer 2: | ACCATTGCTGACCTTCTGGG |