| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.022196 |
| Chromosome: | chromosome 16 |
| Location: | 2075436 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g657300 | CPI1 | Cyclopropyl isomerase-like protein; (1 of 1) 5.5.1.9 - Cycloeucalenol cycloisomerase / Cycloeucalenol--obtusifoliol isomerase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCACATGCATGCAACCTGCTTGCAGCCCCCACGGCGCACCTTGCACCG |
| Internal bar code: | AATTCTTGACTTTCATAATAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2355 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGAGTCCGTGCAAGAGGT |
| Suggested primer 2: | AATCGTGTCGTCCTGGCTTT |