| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.022238 |
| Chromosome: | chromosome 13 |
| Location: | 4442435 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g602550 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATCCTTCAAAAACTCTGCACTCTCCTTCCACCCCTCCTCCCCCATGCT |
| Internal bar code: | TATCCGAAGAATCGGTTTATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3755 |
| LEAP-Seq percent confirming: | 95.2381 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGATGAGGTTGGCGAGGTC |
| Suggested primer 2: | CGACCAAAAGGCAAAAGCGA |