Insertion junction: CLIP2.022240_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.022240
Chromosome:chromosome 1
Location:7654689
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g053700 (1 of 7) PF00027//PF00520 - Cyclic nucleotide-binding domain (cNMP_binding) // Ion transport protein (Ion_trans) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAATGATTGCGACCTCCACGCCAACGCAGGCGAGCCGTGGATGTTCTCC
Internal bar code:TGATCCAGCGGGGAAACGGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:3175
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCCCCACCTGTTTCATGA
Suggested primer 2:CTTCATTGTTGCTTGCCGCT