Insertion junction: CLIP2.022241_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.022241
Chromosome:chromosome 3
Location:5529050
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g185000 GTR8,BGS2,CALS2 Callose synthase 2; (1 of 2) K11000 - callose synthase [EC:2.4.1.-] Glc b1-3 Glc (CALS) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACCGTGGGCGTCGGCGTCAGCAGCAGGCGGGACGGTGCCGTCGGGGCCC
Internal bar code:GCTGGAACTCCTGAACGATTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1614
LEAP-Seq percent confirming:65.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:TTCGTACCACGTGCTACGAC
Suggested primer 2:CCATGAGCTTCTCAGGCGAT