| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.022271 |
| Chromosome: | chromosome 4 |
| Location: | 1114638 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g216000 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGCAATAAGGATGCGGGCAGCGTGAGCATCTTGATGGCTTTGGCCCG |
| Internal bar code: | TACCAGCGGCAATGTAGTGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1015 |
| LEAP-Seq percent confirming: | 73.913 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGATAGGAGGGGATTGCG |
| Suggested primer 2: | GGTGGTTGTCAATGTGTGGC |