| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.022272 |
| Chromosome: | chromosome 5 |
| Location: | 828327 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g246050 | OTU3 | (1 of 1) K13717 - OTU domain-containing protein 3 [EC:3.4.19.12] (OTUD3); OTU-like cysteine protease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGCACCTAGCCTACTCGGACGGCCAGGTAGGGCGGAGGAGAGATCAC |
| Internal bar code: | ATACGTCCAATATGCTAGGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 728 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAAGGCGGGAGTGGACAAA |
| Suggested primer 2: | CCTTCACTCCCCCAACCTTC |