Insertion junction: CLIP2.022274_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.022274
Chromosome:chromosome 6
Location:8220553
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g306400 (1 of 1) 4.4.1.14 - 1-aminocyclopropane-1-carboxylate synthase / S-adenosyl-L-methionine methylthioadenosine-lyase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTACGTAACACACATACATGCACAGACACTCGCACCCAACACAAACACG
Internal bar code:TGGTTGACACCATCATCCACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:4690
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:53
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:AGAGGTTTGTGGTATGGGCG
Suggested primer 2:TTCTTGCATGTGTTGGTGCG