| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.022274 |
| Chromosome: | chromosome 6 |
| Location: | 8220557 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g306400 | (1 of 1) 4.4.1.14 - 1-aminocyclopropane-1-carboxylate synthase / S-adenosyl-L-methionine methylthioadenosine-lyase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGAGAAATCGGTGTGGGTGTTGCAGTCGTCAGCCCAGTGATGAACTTG |
| Internal bar code: | CGGATTTGCACTGGGAAGGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2138 |
| LEAP-Seq percent confirming: | 80.2083 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTTGCATGTGTTGGTGCG |
| Suggested primer 2: | AGAGGTTTGTGGTATGGGCG |