| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.022282 |
| Chromosome: | chromosome 6 |
| Location: | 4665466 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278550 | (1 of 3) PTHR12385//PTHR12385:SF44 - CTL TRANSPORTER // PLASMA-MEMBRANE CHOLINE TRANSPORTER FAMILY PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGGCACGCTGCTGCTGCTGAGCCTGGCGGCGGCGCTGGTGCTGACGG |
| Internal bar code: | TTCACCTGCTACATAAAATCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3171 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGACAGTCAGGACACACG |
| Suggested primer 2: | TACAGCCTGCTGATCCTCCT |