| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.022287 |
| Chromosome: | chromosome 16 |
| Location: | 1581218 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g653700 | (1 of 1) PTHR10972//PTHR10972:SF71 - OXYSTEROL-BINDING PROTEIN-RELATED // OXYSTEROL-BINDING PROTEIN | 5'UTR_intron | |
| Cre16.g653705 | (1 of 1) PTHR14273//PTHR14273:SF0 - FAMILY NOT NAMED // LYR MOTIF-CONTAINING PROTEIN 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCACTCAGTCCAAAGCGCGGGGATGAAGTGCGGAGTATCAGGGCAGGGT |
| Internal bar code: | TTGTTCACACGTGCGACGGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3850 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTGCGACGCTAACCTTGA |
| Suggested primer 2: | ATGTCGGCTTTGGAGTTCGT |