Insertion junction: CLIP2.022290_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.022290
Chromosome:chromosome 6
Location:8176493
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g800751 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTACCTTCTTCCCTTGGCGCAGAAAGAATGGCCCGCGGTCGGTGACCCCC
Internal bar code:CCGTGCTTGATGGGTCTCCCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:3909
LEAP-Seq percent confirming:87.5
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:CGAATTGCTGAAGATGCCGG
Suggested primer 2:CTTCGCGTCAACAACAAGCA