Insertion junction: CLIP2.022291_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.022291
Chromosome:chromosome 12
Location:9710164
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g555378 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTCTCCCCCTCGCCCACACCCGCACCCGCCGGCGGTGGCGCGCCCGCGC
Internal bar code:TTGCGGCTTTTTTCATTGGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:350
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGAATGGGACGTGTGTGT
Suggested primer 2:GTGCCCTCCAAGGTCTTCTC