Insertion junction: CLIP2.022296_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.022296
Chromosome:chromosome 10
Location:2921268
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g439900 HSP70G ER-located HSP110/SSE-like protein; (1 of 1) K09486 - hypoxia up-regulated 1 (HYOU1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCAGCCCGTCCTCCCCTCCCAGCCTCTCCACCCCCCGGGCTCTCCACCT
Internal bar code:TTTCTTTCATTATAGTGTACAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1149
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TCTTTTGCCCTGTTCCCGTT
Suggested primer 2:GAGAGATCTGAGGGGTGGGT