| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.022326 |
| Chromosome: | chromosome 8 |
| Location: | 3402455 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g378000 | (1 of 1) K18749 - protein LSM14 (LSM14, RAP55, SCD6) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGACACTTGTCGCCCTCGTCGCCCTTCACCAGAACTCACCCTTGAAGA |
| Internal bar code: | TTGAAGCCCTTTTTAGCATGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1497 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTGGAAAAATGGGGGAGA |
| Suggested primer 2: | AGTAGCAGCTTCGCCAAACT |