| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.022343 |
| Chromosome: | chromosome 7 |
| Location: | 3288589 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334450 | EFH4 | EF-hand domain protein; (1 of 1) IPR007044 - Cyclodeaminase/cyclohydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTCCATGGCCGACCACCGACCCTTCTGTCAGACTACCGGGGCGTGCG |
| Internal bar code: | CTTGTTTAGACTCCGGTCGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3384 |
| LEAP-Seq percent confirming: | 97.0588 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAAGTTCAGGGTCGGTCG |
| Suggested primer 2: | GCGAAGAAGCGAACGGATTC |