Insertion junction: CLIP2.022351_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.022351
Chromosome:chromosome 4
Location:1217526
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g215250 FAP26 Ankyrin Repeat Flagellar Associated Protein 26; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAAACCCCAAACACACCTTGGTCTTCGCTGTGAGGTACTTCGAAACGCG
Internal bar code:GATATATCGGCTCTTCACGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1022
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GTAACACCCTCCCTCCGTTG
Suggested primer 2:CCTCCATGATGGCTGTCTCC