Insertion junction: CLIP2.022353_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.022353
Chromosome:chromosome 7
Location:2748626
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g331300 AGP3 ADP-glucose pyrophosphorylase large subunit; (1 of 1) PF00132//PF00483 - Bacterial transferase hexapeptide (six repeats) (Hexapep) // Nucleotidyl transferase (NTP_transferase) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTTTGTCGAACACGTAGATGCCGCATGAGGCCAGGAAGGGGCGGCCGT
Internal bar code:TTATACACGCGGTCAGATACAG

Confirmation - LEAP-Seq

LEAP-Seq distance:229
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTGTCCGTACAGCTCTAC
Suggested primer 2:CTGTTGCGGCAATCAACACA