| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.022361 |
| Chromosome: | chromosome 13 |
| Location: | 4137809 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g591250 | EZY20 | (1 of 726) IPR011009 - Protein kinase-like domain; Early zygote expressed protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCGACGCTGCGTCCACGATCGTGCACTGTTAGGTCCTGCAAGTCTGT |
| Internal bar code: | CACGCCGCCAGGCACAGCAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 257 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGCACCAACCTACTTCAC |
| Suggested primer 2: | CACCTCCCCCATACACACAC |