| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.022393 |
| Chromosome: | chromosome 6 |
| Location: | 3473684 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278109 | FAD2B | Putative Oleate/fatty acid desaturase, _-12; (1 of 1) 1.14.15.3 - Alkane 1-monooxygenase / Omega-hydroxylase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCTAGGGATCAAGGTGGGCGGCGAGGGTGTGTGTTTGGGCGGGTGGGG |
| Internal bar code: | TACAGCCATGGTTACGAAAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1282 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAGTCTCTCCCCTAGGCC |
| Suggested primer 2: | GACGGTTTCAGCTGTTGCAG |