Insertion junction: CLIP2.022418_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.022418
Chromosome:chromosome 13
Location:1153542
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g569600 (1 of 5) PF03992 - Antibiotic biosynthesis monooxygenase (ABM) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGACACGCGAACACTCAAGCCTGCGCTTTGCCTTCCGAGTGCCCCCGAAC
Internal bar code:GTGTGATTGTAGTAACTACTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:212
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TGACCACAAGCAACAGGGAG
Suggested primer 2:CCACCCCCGAACATACACTC