| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.022423 |
| Chromosome: | chromosome 14 |
| Location: | 1538187 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g617950 | CAM18 | Calmodulin-like protein; (1 of 5) PTHR23050//PTHR23050:SF90 - CALCIUM BINDING PROTEIN // CENTRIN-3 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCCGGGGTATCTTGAGCAACACCCCGGGGCCGCCAAAGCACGCGGGCC |
| Internal bar code: | CATTGTCACAGGATCGTGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4397 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCCGCATTCGTGTCGTTC |
| Suggested primer 2: | GCCGAGATCGACGAGTTCAT |