Insertion junction: CLIP2.022437_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.022437
Chromosome:chromosome 9
Location:1391865
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g400150 (1 of 2) PF13415//PF13418 - Galactose oxidase, central domain (Kelch_3) // Galactose oxidase, central domain (Kelch_4) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACGCCTGGCTAGAGGTGGAGGTGGAGGGTCCGGCGCCCAGCCCACGTAA
Internal bar code:TTAAGACTGTCTGAGTTGTCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:544
LEAP-Seq percent confirming:90.4762
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CGATATCCAGCCCACCAGTC
Suggested primer 2:GAGATCCGTGAATGCGTTGC