Insertion junction: CLIP2.022437_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.022437
Chromosome:chromosome 9
Location:1391867
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g400150 (1 of 2) PF13415//PF13418 - Galactose oxidase, central domain (Kelch_3) // Galactose oxidase, central domain (Kelch_4) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGGTGGGTTAGGTGGCCAGGGCGACAGAGGGGTTTGCACGAGGGGCTG
Internal bar code:GAAATGTGGTGTGCTCTATCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2053
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:GAGATCCGTGAATGCGTTGC
Suggested primer 2:CGATATCCAGCCCACCAGTC