| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.022452 |
| Chromosome: | chromosome 1 |
| Location: | 4674680 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g032350 | PIG8 | p53-induced protein/etoposide-induced protein 2.4; (1 of 1) K10134 - etoposide-induced 2.4 mRNA (EI24) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGCTGGTTAGCTGCGGCATGTGGGTGCGGGGATGAGAAACGGGGGCAC |
| Internal bar code: | AGTTATTCCCGTGTGATGGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4821 |
| LEAP-Seq percent confirming: | 96.3636 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTAGCCTGAGGGAGGGAG |
| Suggested primer 2: | GCAACGCTGTGATGCTATGG |