| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.022464 |
| Chromosome: | chromosome 4 |
| Location: | 1558745 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213400 | SUB7 | (1 of 1) PTHR10795//PTHR10795:SF419 - PROPROTEIN CONVERTASE SUBTILISIN/KEXIN // SUBFAMILY NOT NAMED; Subtilisin-like protease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCGCGTCATGGATCATCAGCGCCGCCACACCGGACACAGCGGGGGCGG |
| Internal bar code: | TTGAGATGCCTATACCTTGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1500 |
| LEAP-Seq percent confirming: | 81.25 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCGGGATTTGCTTTGTGT |
| Suggested primer 2: | TTGATCTGGAGGGGGAGGAG |