Insertion junction: CLIP2.022474_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.022474
Chromosome:chromosome 13
Location:2156883
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g577750 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGAAAGAAGTCGAGCTGCTCCTCCGATAGTTTGGACAAGACGAACAGCA
Internal bar code:GCAACAAGTGGTAGGTCGCACT

Confirmation - LEAP-Seq

LEAP-Seq distance:2941
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:TGCCCACCTATACGACTGGA
Suggested primer 2:CAGCCAACAGAAAGCAACCC