Insertion junction: CLIP2.022475_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.022475
Chromosome:chromosome 1
Location:1519501
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g008150 RPK1 RNA-polymerase-subunit kinase; (1 of 1) IPR000719//IPR001932//IPR002290//IPR011009 - Protein kinase domain // PPM-type phosphatase domain // Serine/threonine/dual specificity protein kinase, catalytic domain // Protein kinase-like domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGAACATCGTACTCACCAGTGGCGCCAAGCACTCGCCACCTAACGGCAG
Internal bar code:ATTCACTCATCGGGCAAGCGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1889
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGGCATACTCCCACCACG
Suggested primer 2:CCACTGCTACTGCTGCTTCT