Insertion junction: CLIP2.022488_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.022488
Chromosome:chromosome 17
Location:1677532
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g708000 FAP260 Membrane-associated Flagellar Associated Protein 260; (1 of 11) PF13426 - PAS domain (PAS_9) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACCGCAAGCCTCACCGCCCCATGTGATTTGCGTCCCCCGCGGAAGGGAC
Internal bar code:TGGGACGGTAATCAATTCTCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:3140
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TTCAGACGCAACGCAACTTG
Suggested primer 2:GTTCGTGCAAAACCTGGGAC