Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.022488 |
Chromosome: | chromosome 17 |
Location: | 1677532 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g708000 | FAP260 | Membrane-associated Flagellar Associated Protein 260; (1 of 11) PF13426 - PAS domain (PAS_9) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCGCAAGCCTCACCGCCCCATGTGATTTGCGTCCCCCGCGGAAGGGAC |
Internal bar code: | TGGGACGGTAATCAATTCTCCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 3140 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 12 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTCAGACGCAACGCAACTTG |
Suggested primer 2: | GTTCGTGCAAAACCTGGGAC |