| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.022500 |
| Chromosome: | chromosome 8 |
| Location: | 502221 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g359300 | PHO1 | (1 of 1) PTHR11575//PTHR11575:SF29 - 5'-NUCLEOTIDASE-RELATED // SUBFAMILY NOT NAMED; Alkaline phosphatase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCATGAGTACGTACCCGTCAGTGCATCAGTCCACAACAGTGCATCAG |
| Internal bar code: | GAAGACGGTGGTTCCCCAGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2293 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCCACGCTTCTGGACAAG |
| Suggested primer 2: | GTGAGTGGTTGAGAGGGTGG |