Insertion junction: CLIP2.022504_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.022504
Chromosome:chromosome 1
Location:2162913
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g012100 ARS4 Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTGAGGTCAGCCGCCGAACGTGCTGACACCGAACACCTCACGTTGGTG
Internal bar code:GGGAGAAAGTGACCCGTCAATT

Confirmation - LEAP-Seq

LEAP-Seq distance:3708
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GGCTGTGCTGTCAGTACTGT
Suggested primer 2:GACACACACACATGCACACC