Insertion junction: CLIP2.022504_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.022504
Chromosome:chromosome 1
Location:2162913
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g012100 ARS4 Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTAGAACCCCCCATGTCAGAAAGCAATCAGCCAGCGGCAGCAGATGCT
Internal bar code:TTGGGGACTCTTGGAGTGTGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:219
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GACACACACACATGCACACC
Suggested primer 2:GGCTGTGCTGTCAGTACTGT