| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.022509 |
| Chromosome: | chromosome 4 |
| Location: | 3743197 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g229374 | (1 of 352) PF00078 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCCAGCGCCTGTCCCGGCGCCGTCCACCAGTGAGGTCGTCCGCACCA |
| Internal bar code: | AAGGTAATGCATTGTTAGCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3259 |
| LEAP-Seq percent confirming: | 60.7143 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTTGTGTGCGTTGATCTG |
| Suggested primer 2: | AGGAACTGGAGCGATTGGTG |