Insertion junction: CLIP2.022527_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.022527
Chromosome:chromosome 12
Location:8313728
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g551000 (1 of 1) PTHR33966:SF1 - PROTEIN ODR-4 HOMOLOG CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGTCACAGGCGACGGCAGCGGCGCGACGCCAGGTTCTATACCTGCCGAG
Internal bar code:GATCGTGGTTGGTGATCGCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:852
LEAP-Seq percent confirming:95.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTCCTTCCCATGTGCTCT
Suggested primer 2:ATCTACCCACCTGCGTACCT