Insertion junction: CLIP2.022547_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.022547
Chromosome:chromosome 5
Location:876858
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g246300 (1 of 1) PTHR13554//PTHR13554:SF10 - 26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 5-RELATED // 26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 5 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAAGTAAATGGCTCCTTCACCCAACCGCAAACACTCCGCCACGCGTAG
Internal bar code:GTGTGCCTGATACTACTATAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2195
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ATCCGGGGTTACGTAATCGC
Suggested primer 2:CTCTCCATTCCGGGCTTTGT