| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.022558 |
| Chromosome: | chromosome 13 |
| Location: | 799662 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g567050 | (1 of 1) PF00078//PF05869 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) // DNA N-6-adenine-methyltransferase (Dam) (Dam) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGTCGGGCCGTCGGCGTTCGAGTTAGCTTGTTGGCTGCGCTTTGGTTG |
| Internal bar code: | CATTCTCGGAGTGGTATTTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1429 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGTTCCTGAGTGAATGCT |
| Suggested primer 2: | GCCTCTAAGCGAGCTTCCAT |