| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.022561 |
| Chromosome: | chromosome 4 |
| Location: | 1481553 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213904 | Mitogen-Activated Protein Kinase 3; (1 of 1) K02208 - cyclin-dependent kinase 8/11 [EC:2.7.11.22 2.7.11.23] (CDK8_11) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGACGGTAGACCATCTTGGGGTGGGGTGGTTGGTGGGAAGGGTTGGGT |
| Internal bar code: | CTCACACCTCAGGGATCCAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2725 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAAATCATGCTGCTGAGG |
| Suggested primer 2: | CAGTGTATGCGATGCGTGTG |