| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.022585 |
| Chromosome: | chromosome 9 |
| Location: | 5576303 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409650 | (1 of 18) PF12906 - RING-variant domain (RINGv) | gene_edge/mRNA_edge/CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAGCACACTTGACAGCGCCTCGACGAGCGCGGCCACAATCGTCGGTTC |
| Internal bar code: | GTGTGCAGAGTTCTATAGTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2549 |
| LEAP-Seq percent confirming: | 86.9565 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTAAACGCGCGATCTGAC |
| Suggested primer 2: | CTCTGCCCATGCTTCTTCCA |