| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.022600 |
| Chromosome: | chromosome 2 |
| Location: | 640410 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g077700 | (1 of 2) PTHR22957//PTHR22957:SF148 - TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN // RE26521P | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGTGGCGGAATGTGTGTGTGTGTGGGGGGGGGGGGGGTAGTGCGGTGC |
| Internal bar code: | TGTTTTTGAATTGCAAGAAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 155 |
| LEAP-Seq percent confirming: | 6.66667 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTCCTCGCTCTTTTGCA |
| Suggested primer 2: | CTGTGTCCCATCCCCCTTTC |