Insertion junction: CLIP2.022654_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.022654
Chromosome:chromosome 8
Location:3990814
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g382545 CNX7 Molybdenum cofactor biosynthesis protein; (1 of 2) 2.8.1.12 - Molybdopterin synthase / MPT synthase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGGAGGACCTGCTCGCACAGATTATAGCTGCGCACCCGGCGCTGGATA
Internal bar code:GTGCGCGCAGGTAGGCAACGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2626
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCACGTGAATCACCACTA
Suggested primer 2:CAATGAATAGGGCGCAACCG