Insertion junction: CLIP2.022674_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.022674
Chromosome:chromosome 17
Location:4056741
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g728650 FAP196 (1 of 2) PTHR13720//PTHR13720:SF14 - WD-40 REPEAT PROTEIN // WD REPEAT-CONTAINING PROTEIN 16; Flagellar Associated Protein 196 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGCGGTCCAGGGTCACAGTCACCACCGACACCTGTTGACGCAGTGTCC
Internal bar code:GGTTCATGGTTTGACGCACGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2441
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:CCACCAACTGTCGGATCCAA
Suggested primer 2:GGCACGATCTCCACAAGTCA