Insertion junction: CLIP2.022675_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.022675
Chromosome:chromosome 11
Location:2100214
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467751 (1 of 4) K06067 - histone deacetylase 1/2 (HDAC1_2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTATCATAAACAATCGCACTCCCGCGTCGCAGCCCCGCACCTGCAGGCC
Internal bar code:AACTTTGACTGTGATGGATTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:863
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGACGACGAGGAGGAAGATG
Suggested primer 2:TCAAGCCACTCGTCACTCAC