| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.022688 |
| Chromosome: | chromosome 2 |
| Location: | 8297412 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g143250 | IDH2 | (1 of 2) 1.1.1.41 - Isocitrate dehydrogenase (NAD(+)) / Nicotinamide adenine dinucleotide isocitrate dehydrogenase; Isocitrate dehydrogenase, NAD-dependent | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAGAACTAGTGCGTGCGCGGCCTTGGCGACAACCTTGACAGTTGTGCG |
| Internal bar code: | CGATGTTACTTGTGGTATCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2321 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGAGCTGGACCTTTACGC |
| Suggested primer 2: | CGAGATGATGTCGCCGTACA |