| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.022689 |
| Chromosome: | chromosome 2 |
| Location: | 251510 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g800158 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCAGCGTAAGCCTCACACTCAAGGAGTACGTGCCGTTCATCCTCAACT |
| Internal bar code: | CGGTCCATGAAACCTGAGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4130 |
| LEAP-Seq percent confirming: | 87.234 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTCCGTGAGCGCATGATG |
| Suggested primer 2: | TGCTTGGGGGTTACGACTTC |