Insertion junction: CLIP2.022694_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.022694
Chromosome:chromosome 10
Location:2440030
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g435350 ESU1,ESU1a Endosulfine cAMP-regulated phosphoprotein; (1 of 2) PF04667 - cAMP-regulated phosphoprotein/endosulfine conserved region (Endosulfine) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCTCGGGGCTGCCTCAGCGGCGGGTCCGTGTACTAAGAAAACTTGACA
Internal bar code:ATTTGGATAGGGTGGGTCAACA

Confirmation - LEAP-Seq

LEAP-Seq distance:2369
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:48
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:ATGCACACGGAGGTCAGAAG
Suggested primer 2:GTCGCGTAGGCCTACTTCTC