| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.022695 |
| Chromosome: | chromosome 9 |
| Location: | 2699077 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391753 | CDS | ||
| Cre09.g801023 | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCTGAGTTCAGTACGTCATATGCATATGCATAATCCATCCCTCTATTT |
| Internal bar code: | ATAGAATAAGCCGTCACCGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2883 |
| LEAP-Seq percent confirming: | 95.8333 |
| LEAP-Seq n confirming: | 69 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCTTCTCCCTAGAGCTGT |
| Suggested primer 2: | CCTCTGGTCAGCCCTTTCAG |