Insertion junction: CLIP2.022707_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.022707
Chromosome:chromosome 1
Location:4082125
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g027050 (1 of 3) PF11527 - The ARF-like 2 binding protein BART (ARL2_Bind_BART) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCGCACCTCCAGCTTCTCCAACATCTCCATTTGTGACATTCTTGAGCG
Internal bar code:TTATTGGTGAGGTTAATCTAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2243
LEAP-Seq percent confirming:90.9091
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:CCGATTCACGCGTAAAGCTG
Suggested primer 2:AACTCCTCCAGTTGCTGCTC