| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.022722 |
| Chromosome: | chromosome 12 |
| Location: | 5166499 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g526850 | HEL56 | (1 of 1) K14810 - ATP-dependent RNA helicase DDX56/DBP9 [EC:3.6.4.13] (DDX56, DBP9); DEAD box ATP-dependent RNA helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGCTCACCCTGCCTGCACCCACCCACCCACCACCATCACCACCATCAC |
| Internal bar code: | AAAGCTTACTACCTTTCTCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1380 |
| LEAP-Seq percent confirming: | 97.1429 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTCTATTCCGTCCTCCAC |
| Suggested primer 2: | GAGGCCTCACAGCATCCTTT |