Insertion junction: CLIP2.022733_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.022733
Chromosome:chromosome 7
Location:1012663
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g319600 FAE3 (1 of 3) K15397 - 3-ketoacyl-CoA synthase (KCS); Putative 3-keto-acyl-CoA synthase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTATGAGAAATAACGCGGGATGAGAAGGGGCACGAGTGCATGTTGCGCAT
Internal bar code:TGTTACGGGGATTCGAGAACCT

Confirmation - LEAP-Seq

LEAP-Seq distance:879
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTATGCACTTGCTCACGT
Suggested primer 2:ACCCTTCCCCTCATCTCTCC